The preparation and function of the hypertensin-converting enzyme

LT Skeggs Jr, JR Kahn… - The Journal of …, 1956 - ncbi.nlm.nih.gov
LT Skeggs Jr, JR Kahn, NP Shumway
The Journal of experimental medicine, 1956ncbi.nlm.nih.gov
It has been shown by use of isolated, perfused rat kidneys that hypertensin II is a potent
vasoconstrictor substance while hypertensin I is not. Hence it would appear that in intact
animals the pressor activity of hypertensin I results from its rapid conversion to hypertensin II.
An enzyme which effects this conversion has been procured from horse plasma in a
semipurified form by means of ammonium sulfate fractionation and isoelectric precipitation.
A method is described for estimating the activity of the enzyme. An example of the use of the …
Abstract
It has been shown by use of isolated, perfused rat kidneys that hypertensin II is a potent vasoconstrictor substance while hypertensin I is not. Hence it would appear that in intact animals the pressor activity of hypertensin I results from its rapid conversion to hypertensin II. An enzyme which effects this conversion has been procured from horse plasma in a semipurified form by means of ammonium sulfate fractionation and isoelectric precipitation. A method is described for estimating the activity of the enzyme. An example of the use of the preparation in converting purified hypertensin I to hypertensin II has been described.
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