Conjugation of magnetic beads for immunopurification of protein complexes
IM Cristea, BT Chait - Cold Spring Harbor Protocols, 2011 - cshprotocols.cshlp.org
IM Cristea, BT Chait
Cold Spring Harbor Protocols, 2011•cshprotocols.cshlp.orgConjugation of Magnetic Beads for Immunopurification of Protein Complexes Page 1
Protocol Conjugation of Magnetic Beads for Immunopurification of Protein Complexes
Ileana M. Cristea and Brian T. Chait INTRODUCTION Knowledge of the composition of
protein complexes provides key insights into their functions. Immunoaffinity purification
provides an effective means for isolating protein complexes and elucidating their
composition. Immunoisolation is achieved with antibodies directed either specifically …
Protocol Conjugation of Magnetic Beads for Immunopurification of Protein Complexes
Ileana M. Cristea and Brian T. Chait INTRODUCTION Knowledge of the composition of
protein complexes provides key insights into their functions. Immunoaffinity purification
provides an effective means for isolating protein complexes and elucidating their
composition. Immunoisolation is achieved with antibodies directed either specifically …
METHOD
It is best to carry out Steps 1–5 in the afternoon (∼ 4: 00 PM) and wash the conjugated beads the next morning.
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