In vitro methods for investigating desmoplakin–intermediate filament interactions and their role in adhesive strength
Publisher Summary This chapter describes two very different approaches for investigating
intermediate filament–Desmoplakins (IF–DP) interactions—in vitro purification/
cosedimentation and yeast three-hybrid (Y3H) analyses—and discusses functional assays
for determining the mechanical properties of epithelial cell sheets harboring mutant DPs that
compromise the IF–desmosome connection. The various approaches that have been used
to address the question of IF–DP interactions have provided data that are frequently in …
intermediate filament–Desmoplakins (IF–DP) interactions—in vitro purification/
cosedimentation and yeast three-hybrid (Y3H) analyses—and discusses functional assays
for determining the mechanical properties of epithelial cell sheets harboring mutant DPs that
compromise the IF–desmosome connection. The various approaches that have been used
to address the question of IF–DP interactions have provided data that are frequently in …