3-Hydroxy-3-methylglutaric, adipic, and 2-oxoglutaric acids measured by HPLC in the plasma from diabetic patients
G Lippe, R Trevisan, R Nosadini, R Fabris, R Deana - Clinical Biochemistry, 1987 - Elsevier
G Lippe, R Trevisan, R Nosadini, R Fabris, R Deana
Clinical Biochemistry, 1987•ElsevierA method for the measurement of organic acids in human plasma is presented. The
analytical procedure consists of plasma protein precipitation with acetonitrile, acid extraction
by chromatography through a DEAE-cellulose column eluted with 100 mM perchloric acid,
HPLC by cation-exchange column Aminex HPX-87 eluted with 6.5 mM sulfuric acid. Adipic,
3-hydroxy-3-methylglutaric, 2-oxoglutaric, and citric acids were determined in the plasma of
diabetic patients. The concentrations of all the measured acids, but particularly those of …
analytical procedure consists of plasma protein precipitation with acetonitrile, acid extraction
by chromatography through a DEAE-cellulose column eluted with 100 mM perchloric acid,
HPLC by cation-exchange column Aminex HPX-87 eluted with 6.5 mM sulfuric acid. Adipic,
3-hydroxy-3-methylglutaric, 2-oxoglutaric, and citric acids were determined in the plasma of
diabetic patients. The concentrations of all the measured acids, but particularly those of …
A method for the measurement of organic acids in human plasma is presented. The analytical procedure consists of plasma protein precipitation with acetonitrile, acid extraction by chromatography through a DEAE-cellulose column eluted with 100 mM perchloric acid, HPLC by cation-exchange column Aminex HPX-87 eluted with 6.5 mM sulfuric acid. Adipic, 3-hydroxy-3-methylglutaric, 2-oxoglutaric, and citric acids were determined in the plasma of diabetic patients. The concentrations of all the measured acids, but particularly those of adipic and 3-hydroxy-3-methylglutaric acids, were significantly higher than those of healthy controls. These results suggest that in diabetics the ω-oxidation of fatty acids is enhanced.
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