Dectin-2 mediates Th2 immunity through the generation of cysteinyl leukotrienes

NA Barrett, OM Rahman, JM Fernandez… - Journal of Experimental …, 2011 - rupress.org
NA Barrett, OM Rahman, JM Fernandez, MW Parsons, W Xing, KF Austen, Y Kanaoka
Journal of Experimental Medicine, 2011rupress.org
The innate signaling pathways for Th2 immunity activated by inhaled antigens are not well
defined. We previously identified Dectin-2 as a receptor for glycans in allergen extracts from
the house dust mite Dermatophagoides farinae (Df) that mediates cysteinyl leukotriene (cys-
LT) generation from pulmonary CD11c+ cells and from GM-CSF–cultured bone marrow cells
(BMCsGM-CSF). Using lentiviral knockdown of Dectin-2 in BMCsGM-CSF and adoptive
transfer of Df-pulsed BMCsGM-CSF to sensitize naive mice, we now report that Dectin-2 is …
The innate signaling pathways for Th2 immunity activated by inhaled antigens are not well defined. We previously identified Dectin-2 as a receptor for glycans in allergen extracts from the house dust mite Dermatophagoides farinae (Df) that mediates cysteinyl leukotriene (cys-LT) generation from pulmonary CD11c+ cells and from GM-CSF–cultured bone marrow cells (BMCsGM-CSF). Using lentiviral knockdown of Dectin-2 in BMCsGM-CSF and adoptive transfer of Df-pulsed BMCsGM-CSF to sensitize naive mice, we now report that Dectin-2 is critical for the development of Df-elicited eosinophilic and neutrophilic pulmonary inflammation and Th2 cytokine generation in the lungs and restimulated lymph nodes. Sensitization with Df-pulsed BMCsGM-CSF from LTC4 synthase (LTC4S)–deficient mice or type 1 cys-LT receptor (CysLT1R)–deficient mice demonstrated that both proteins were required for Df-elicited eosinophilic pulmonary inflammation and Th2 cytokine generation in the lungs and restimulated lymph nodes. Direct sensitization and challenge of Ltc4s−/− and Cysltr1−/− mice confirmed that cys-LTs mediate these parameters of Df-elicited Th2 pulmonary inflammation. Thus, the Dectin-2–cys-LT pathway is critical for the induction of Th2 immunity to a major allergen, in part through CysLT1R. These findings identify a previously unrecognized link between a myeloid C-type lectin receptor and Th2 immunity.
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