Real-time selective sequencing using nanopore technology
M Loose, S Malla, M Stout - Nature methods, 2016 - nature.com
M Loose, S Malla, M Stout
Nature methods, 2016•nature.comAbstract The Oxford Nanopore Technologies MinION sequencer enables the selection of
specific DNA molecules for sequencing by reversing the driving voltage across individual
nanopores. To directly select molecules for sequencing, we used dynamic time warping to
match reads to reference sequences. We demonstrate our open-source Read Until software
in real-time selective sequencing of regions within small genomes, individual amplicon
enrichment and normalization of an amplicon set.
specific DNA molecules for sequencing by reversing the driving voltage across individual
nanopores. To directly select molecules for sequencing, we used dynamic time warping to
match reads to reference sequences. We demonstrate our open-source Read Until software
in real-time selective sequencing of regions within small genomes, individual amplicon
enrichment and normalization of an amplicon set.
Abstract
The Oxford Nanopore Technologies MinION sequencer enables the selection of specific DNA molecules for sequencing by reversing the driving voltage across individual nanopores. To directly select molecules for sequencing, we used dynamic time warping to match reads to reference sequences. We demonstrate our open-source Read Until software in real-time selective sequencing of regions within small genomes, individual amplicon enrichment and normalization of an amplicon set.
nature.com