Sickle cell imaging flow cytometry assay (SIFCA)
Imaging Flow Cytometry: Methods and Protocols, 2016•Springer
Hemoglobin S polymerization under hypoxic conditions in sickle cell disorders causes
characteristic shape changes to human red blood cells. Previous sickling assays used to
investigate the efficacy of novel agents to treat these disorders are laborious and observer
dependent. Here, we describe a partially automated, high-throughput sickling assay using
imaging flow cytometry.
characteristic shape changes to human red blood cells. Previous sickling assays used to
investigate the efficacy of novel agents to treat these disorders are laborious and observer
dependent. Here, we describe a partially automated, high-throughput sickling assay using
imaging flow cytometry.
Abstract
Hemoglobin S polymerization under hypoxic conditions in sickle cell disorders causes characteristic shape changes to human red blood cells. Previous sickling assays used to investigate the efficacy of novel agents to treat these disorders are laborious and observer dependent. Here, we describe a partially automated, high-throughput sickling assay using imaging flow cytometry.
Springer