Localization of lipoprotein lipase mRNA in selected rat tissues.
IJ Goldberg, DR Soprano, ML Wyatt, TM Vanni… - Journal of lipid …, 1989 - ASBMB
Measurements of enzymatic activity have demonstrated that lipoprotein lipase (LPL), the
principal enzyme responsible for hydrolysis of circulating triglyceride, is present in a number
of tissues including brain, kidney, and adrenal gland. To determine the sites of synthesis of
LPL in these tissues, in situ hybridization studies were performed using a non-sense 35S-
labeled RNA probe produced from a 624-bp mouse LPL cDNA fragment. Control studies
were performed with a sense RNA strand. Using 5-10-micron sections of 5-day-old rat brain …
principal enzyme responsible for hydrolysis of circulating triglyceride, is present in a number
of tissues including brain, kidney, and adrenal gland. To determine the sites of synthesis of
LPL in these tissues, in situ hybridization studies were performed using a non-sense 35S-
labeled RNA probe produced from a 624-bp mouse LPL cDNA fragment. Control studies
were performed with a sense RNA strand. Using 5-10-micron sections of 5-day-old rat brain …