[PDF][PDF] Widespread predicted nonsense-mediated mRNA decay of alternatively-spliced transcripts of human normal and disease genes

RE Green, BP Lewis, RT Hillman, M Blanchette… - …, 2003 - ocf.berkeley.edu
RE Green, BP Lewis, RT Hillman, M Blanchette, LF Lareau, AT Garnett, DC Rio, SE Brenner
Bioinformatics, 2003ocf.berkeley.edu
We have recently shown that a third of reliably-inferred alternative mRNA isoforms are
candidates for nonsensemediated mRNA decay (NMD), an mRNA surveillance system
(Lewis et al., 2003, Proc. Natl Acad. Sci. USA, 100, 189–192). Rather than being translated
to yield protein, these transcripts are expected to be degraded and may be subject to
regulated unproductive splicing and translation (RUST). Our initial experimental studies are
consistent with these predictions and suggest an unappreciated role for NMD in several …
Abstract
We have recently shown that a third of reliably-inferred alternative mRNA isoforms are candidates for nonsensemediated mRNA decay (NMD), an mRNA surveillance system (Lewis et al., 2003, Proc. Natl Acad. Sci. USA, 100, 189–192). Rather than being translated to yield protein, these transcripts are expected to be degraded and may be subject to regulated unproductive splicing and translation (RUST). Our initial experimental studies are consistent with these predictions and suggest an unappreciated role for NMD in several human diseases. Contact: brenner@ compbio. berkeley. edu
ocf.berkeley.edu