Molecular Assessment, Drug‐Resistant Profile, and Spacer Oligonucleotide Typing (Spoligotyping) of Mycobacterium tuberculosis Strains From Tamaulipas, México
V Bocanegra‐García, E Garza‐González… - Journal of clinical …, 2014 - Wiley Online Library
V Bocanegra‐García, E Garza‐González, WL Cruz‐Pulido, YL Guevara‐Molina…
Journal of clinical laboratory analysis, 2014•Wiley Online LibraryBackground Tuberculosis remains a serious global health problem involving one‐third of the
world population. A wide diversity of Mycobacterium tuberculosis strains cause about 1.5
million deaths/year worldwide, but in developing countries, the genetic diversity of M.
tuberculosis strains remains largely unknown. We conducted a first insight into the
population diversity of M. tuberculosis strains from Tamaulipas, Mexico. Methods Seventy‐
two M. tuberculosis strains were identified and genetic diversity determined by …
world population. A wide diversity of Mycobacterium tuberculosis strains cause about 1.5
million deaths/year worldwide, but in developing countries, the genetic diversity of M.
tuberculosis strains remains largely unknown. We conducted a first insight into the
population diversity of M. tuberculosis strains from Tamaulipas, Mexico. Methods Seventy‐
two M. tuberculosis strains were identified and genetic diversity determined by …
Background
Tuberculosis remains a serious global health problem involving one‐third of the world population. A wide diversity of Mycobacterium tuberculosis strains cause about 1.5 million deaths/year worldwide, but in developing countries, the genetic diversity of M. tuberculosis strains remains largely unknown. We conducted a first insight into the population diversity of M. tuberculosis strains from Tamaulipas, Mexico.
Methods
Seventy‐two M. tuberculosis strains were identified and genetic diversity determined by spoligotyping. Drug sensibility testing and punctual mutations in inhA, ahpC, rpoB, and katG genes were assessed.
Results
Spoligotyping analysis showed a higher prevalence of LAM9 > T1 > Haarlem3 subfamilies among 53 spoligotype patterns. Unexpectedly, five Beijing strains conforming four unique spoligopatterns were recovered. The more frequently isolated strains (LAM9 and T1), but none of the Beijing strains, were found resistant to INH or RIF. Also, no drug resistance was found among Haarlem3 isolates. The katG315 gene mutation was found in 83% of INH‐resistant strains, whereas rpoB526 were associated in only 43% of RIF M. tuberculosis drug‐resistant strains.
Conclusions
This and other studies report a high rate of orphan spoligotypes, which highlights the need for genotyping implementation as a routine technique for better understanding of M. tuberculosis strains in developing countries such as Mexico.
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