[27] Enzyme-linked immunoabsorbent assay (ELISA) and radioimmunoassay (RIA) for the vitamin D-dependent 28,000 dalton calcium-binding protein
BE Miller, AW Norman - Methods in Enzymology, 1983 - Elsevier
Publisher Summary This chapter discusses two sensitive and specific assay methods for the
quantitation of intestinal calcium-binding protein (CaBP) in the chick intestine: enzyme-
linked immunoabsorbent assay (ELISA) and a radioimmunoassay (RIA). Both ELISA and
RIA use antisera produced in the rabbit against highly purified chick intestinal CaBP. The
primary difference between the ELISA and the RIA is the use of an enzyme-linked second
antibody in ELISA rather than 125 I iodination of CaBP in RIA. RIA is based on the …
quantitation of intestinal calcium-binding protein (CaBP) in the chick intestine: enzyme-
linked immunoabsorbent assay (ELISA) and a radioimmunoassay (RIA). Both ELISA and
RIA use antisera produced in the rabbit against highly purified chick intestinal CaBP. The
primary difference between the ELISA and the RIA is the use of an enzyme-linked second
antibody in ELISA rather than 125 I iodination of CaBP in RIA. RIA is based on the …
