In vivo generation of human dendritic cell subsets by Flt3 ligand
E Maraskovsky, E Daro, E Roux… - Blood, The Journal …, 2000 - ashpublications.org
E Maraskovsky, E Daro, E Roux, M Teepe, CR Maliszewski, J Hoek, D Caron, ME Lebsack…
Blood, The Journal of the American Society of Hematology, 2000•ashpublications.orgDendritic cells (DCs) represent a family of ontogenically distinct leukocytes involved in
immune response regulation. The ability of DCs to stimulate T-cell immunity has led to their
use as vectors for immunotherapy vaccines. However, it is unclear whether and to what
degree in vitro–generated DCs are representative of DCs that develop in vivo. Treatment of
mice with human Flt3 ligand (FL) dramatically increases the number of DCs. We report here
that administration of FL to healthy human volunteers increased the number of circulating …
immune response regulation. The ability of DCs to stimulate T-cell immunity has led to their
use as vectors for immunotherapy vaccines. However, it is unclear whether and to what
degree in vitro–generated DCs are representative of DCs that develop in vivo. Treatment of
mice with human Flt3 ligand (FL) dramatically increases the number of DCs. We report here
that administration of FL to healthy human volunteers increased the number of circulating …
Abstract
Dendritic cells (DCs) represent a family of ontogenically distinct leukocytes involved in immune response regulation. The ability of DCs to stimulate T-cell immunity has led to their use as vectors for immunotherapy vaccines. However, it is unclear whether and to what degree in vitro–generated DCs are representative of DCs that develop in vivo. Treatment of mice with human Flt3 ligand (FL) dramatically increases the number of DCs. We report here that administration of FL to healthy human volunteers increased the number of circulating CD11c+ IL-3Rlow DC (mean 44-fold) and CD11c− IL-3Rhigh DC precursors (mean 12-fold). Moreover, the CD11c+ DCs were efficient stimulators of T cells in vitro. Thus, FL can expand the number of circulating, functionally competent human DCs in vivo.
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