A GDP/GTP exchange protein for the Rab3 small G protein family up-regulates a postdocking step of synaptic exocytosis in central synapses
K Yamaguchi, M Tanaka, A Mizoguchi, Y Hirata… - Proceedings of the …, 2002 - pnas.org
K Yamaguchi, M Tanaka, A Mizoguchi, Y Hirata, H Ishizaki, K Kaneko, J Miyoshi, Y Takai
Proceedings of the National Academy of Sciences, 2002•pnas.orgThe Rab3 GDP/GTP exchange protein (Rab3 GEP) activates the Rab3 small GTP-binding
protein (G protein) family, including Rab3A that is an important member controlling synaptic
vesicle trafficking. Here, we examined the role of Rab3 GEP in regulating neurotransmitter
release in autapses of mouse hippocampal neurons in culture. The release probability was
markedly reduced in Rab3 GEP−/− neurons, whereas the readily releasable pool size was
not different between WT and Rab3 GEP−/− neurons, indicating that Rab3 GEP up-regulates …
protein (G protein) family, including Rab3A that is an important member controlling synaptic
vesicle trafficking. Here, we examined the role of Rab3 GEP in regulating neurotransmitter
release in autapses of mouse hippocampal neurons in culture. The release probability was
markedly reduced in Rab3 GEP−/− neurons, whereas the readily releasable pool size was
not different between WT and Rab3 GEP−/− neurons, indicating that Rab3 GEP up-regulates …
The Rab3 GDP/GTP exchange protein (Rab3 GEP) activates the Rab3 small GTP-binding protein (G protein) family, including Rab3A that is an important member controlling synaptic vesicle trafficking. Here, we examined the role of Rab3 GEP in regulating neurotransmitter release in autapses of mouse hippocampal neurons in culture. The release probability was markedly reduced in Rab3 GEP−/− neurons, whereas the readily releasable pool size was not different between WT and Rab3 GEP−/− neurons, indicating that Rab3 GEP up-regulates a postdocking step of synaptic exocytosis. Because Rab3A reportedly down-regulates Ca2+-triggered fusion of synaptic vesicles, these results provide evidence for a role of Rab3 GEP in the postdocking process distinct from Rab3A activation.
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