MADD, a splice variant of IG20, is indispensable for MAPK activation and protection against apoptosis upon tumor necrosis factor-α treatment
BRV Kurada, LC Li, N Mulherkar… - Journal of Biological …, 2009 - jbc.org
We investigated the physiological role of endogenous MAPK-activating death domain-
containing protein (MADD), a splice variant of the IG20 gene, that can interact with TNFR1 in
tumor necrosis factor-α (TNFα)-induced activation of NF-κB, MAPK, ERK1/2, JNK, and p38.
Using exon-specific short hairpin RNAs expressing lentiviruses, we knocked down the
expression of all IG20 splice variants or MADD, which is overexpressed in cancer cells.
Abrogation of MADD expression rendered cells highly susceptible to TNFα-induced …
containing protein (MADD), a splice variant of the IG20 gene, that can interact with TNFR1 in
tumor necrosis factor-α (TNFα)-induced activation of NF-κB, MAPK, ERK1/2, JNK, and p38.
Using exon-specific short hairpin RNAs expressing lentiviruses, we knocked down the
expression of all IG20 splice variants or MADD, which is overexpressed in cancer cells.
Abrogation of MADD expression rendered cells highly susceptible to TNFα-induced …
