Cystic Fibrosis (CF) is a lethal autosomal recessive disease caused by mutations in the
gene encoding the cystic fibrosis transmembrane conductance regulator (CFTR). The most
common mutation is the deletion of phenylalanine residue at position 508 (ΔF508). Here we
report the production of CFTR-ΔF508 rabbits by CRISPR/Cas9-mediated gene editing. After
microinjection and embryo transfer, 77 kits were born, of which five carried the ΔF508
mutation. To confirm the germline transmission, one male ΔF508 founder was bred with two …

Cystic Fibrosis (CF) is a lethal autosomal recessive disease caused by mutations in the
gene encoding the cystic fibrosis transmembrane conductance regulator (CFTR). The most
common mutation is the deletion of phenylalanine residue at position 508 (AF508). Here we
report the production of CFTR-AF508 rabbits by CRISPR/Cas9-mediated gene editing. After
microinjection and embryo transfer, 77 kits were born, of which five carried the AF508
mutation. To confirm the germline transmission, one male AF508 founder was bred with two …