Analysis of the effect of various decalcification agents on the quantity and quality of nucleic acid (DNA and RNA) recovered from bone biopsies
VM Singh, RC Salunga, VJ Huang, Y Tran… - Annals of diagnostic …, 2013 - Elsevier
Molecular studies are part of standard care for cancer patients. Bone, a common and
sometimes sole site of metastasis, requires decalcification for morphological examination.
Many commonly used decalcification agents contain strong acids that degrade nucleic
acids. The paradigm shift in oncology, with biomarker targeted therapy and gene expression
profiling analysis, requires sufficient nucleic acid recovery from bone biopsy specimens. We
systematically studied the effects of a spectrum of decalcification agents on the quantity and …
sometimes sole site of metastasis, requires decalcification for morphological examination.
Many commonly used decalcification agents contain strong acids that degrade nucleic
acids. The paradigm shift in oncology, with biomarker targeted therapy and gene expression
profiling analysis, requires sufficient nucleic acid recovery from bone biopsy specimens. We
systematically studied the effects of a spectrum of decalcification agents on the quantity and …
