Coomassie staining as loading control in Western blot analysis
C Welinder, L Ekblad - Journal of proteome research, 2011 - ACS Publications
C Welinder, L Ekblad
Journal of proteome research, 2011•ACS PublicationsIn Western blotting, immunodetection of housekeeping proteins is routinely performed to
detect differences in electrophoresis loading. The present work describes a much faster and
simpler protein staining method, which is compatible with ordinary blocking conditions. In
addition, the method can be used after immunodetection with superior linearity compared to
ordinary staining methods. After immunoblotting and staining, protein bands can be further
identified using peptide mass fingerprinting.
detect differences in electrophoresis loading. The present work describes a much faster and
simpler protein staining method, which is compatible with ordinary blocking conditions. In
addition, the method can be used after immunodetection with superior linearity compared to
ordinary staining methods. After immunoblotting and staining, protein bands can be further
identified using peptide mass fingerprinting.
In Western blotting, immunodetection of housekeeping proteins is routinely performed to detect differences in electrophoresis loading. The present work describes a much faster and simpler protein staining method, which is compatible with ordinary blocking conditions. In addition, the method can be used after immunodetection with superior linearity compared to ordinary staining methods. After immunoblotting and staining, protein bands can be further identified using peptide mass fingerprinting.
ACS Publications