An optimized assay for the enumeration of antigen-specific memory B cells in different compartments of the human body
Y Cao, M Gordic, S Kobold, N Lajmi, S Meyer… - Journal of …, 2010 - Elsevier
OBJECT: In the framework of our current study we set out to develop an optimized assay for
the quantification of antigen-specific B cells in different compartments of the human body.
METHODS: Mononuclear cells (MNC) derived from the peripheral blood, bone marrow (BM),
or human tonsils were incubated with different combinations of stimuli. The stimulated cells
and culture supernatants were then applied to IgG-ELISPOT and ELISA read-out assays and
tetanus toxoid (TT)-specific B cell responses were quantified. RESULTS: We found that a …
the quantification of antigen-specific B cells in different compartments of the human body.
METHODS: Mononuclear cells (MNC) derived from the peripheral blood, bone marrow (BM),
or human tonsils were incubated with different combinations of stimuli. The stimulated cells
and culture supernatants were then applied to IgG-ELISPOT and ELISA read-out assays and
tetanus toxoid (TT)-specific B cell responses were quantified. RESULTS: We found that a …