Isolation of ubiquitylated proteins using tandem ubiquitin-binding entities
Ubiquitin family modifiers and the proteasome: reviews and protocols, 2012•Springer
Studying postubiquitylation events has always been a difficult task due to the labile nature of
these posttranslational modifications. When utilized in tandem, ubiquitin-binding entities
(TUBEs) not only increase up to thousand times the affinity for poly-ubiquitin chains but also
protect ubiquitylated proteins from the action of the proteasome and de-ubiquitylating
enzymes.
these posttranslational modifications. When utilized in tandem, ubiquitin-binding entities
(TUBEs) not only increase up to thousand times the affinity for poly-ubiquitin chains but also
protect ubiquitylated proteins from the action of the proteasome and de-ubiquitylating
enzymes.
Abstract
Studying postubiquitylation events has always been a difficult task due to the labile nature of these posttranslational modifications. When utilized in tandem, ubiquitin-binding entities (TUBEs) not only increase up to thousand times the affinity for poly-ubiquitin chains but also protect ubiquitylated proteins from the action of the proteasome and de-ubiquitylating enzymes.
Springer