Ubiquitination of β-arrestin links seven-transmembrane receptor endocytosis and ERK activation
SK Shenoy, LS Barak, K Xiao, S Ahn… - Journal of Biological …, 2007 - jbc.org
β-Arrestin2 and its ubiquitination play crucial roles in both internalization and signaling of
seven-transmembrane receptors (7TMRs). To understand the connection between
ubiquitination and the endocytic and signaling functions of β-arrestin, we generated a β-
arrestin2 mutant that is defective in ubiquitination (β-arrestin2 0K), by mutating all of the
ubiquitin acceptor lysines to arginines and compared its properties with the wild type and a
stably ubiquitinated β-arrestin2-ubiquitin (Ub) chimera. In vitro translated β-arrestin2 and β …
seven-transmembrane receptors (7TMRs). To understand the connection between
ubiquitination and the endocytic and signaling functions of β-arrestin, we generated a β-
arrestin2 mutant that is defective in ubiquitination (β-arrestin2 0K), by mutating all of the
ubiquitin acceptor lysines to arginines and compared its properties with the wild type and a
stably ubiquitinated β-arrestin2-ubiquitin (Ub) chimera. In vitro translated β-arrestin2 and β …
