[HTML][HTML] Nedd4 mediates agonist-dependent ubiquitination, lysosomal targeting, and degradation of the β2-adrenergic receptor
SK Shenoy, K Xiao, V Venkataramanan… - Journal of Biological …, 2008 - ASBMB
Agonist-stimulated β 2-adrenergic receptor (β 2 AR) ubiquitination is a major factor that
governs both lysosomal trafficking and degradation of internalized receptors, but the identity
of the E3 ubiquitin ligase regulating this process was unknown. Among the various
catalytically inactive E3 ubiquitin ligase mutants that we tested, a dominant negative Nedd4
specifically inhibited isoproterenol-induced ubiquitination and degradation of the β 2 AR in
HEK-293 cells. Moreover, siRNA that down-regulates Nedd4 expression inhibited β 2 AR …
governs both lysosomal trafficking and degradation of internalized receptors, but the identity
of the E3 ubiquitin ligase regulating this process was unknown. Among the various
catalytically inactive E3 ubiquitin ligase mutants that we tested, a dominant negative Nedd4
specifically inhibited isoproterenol-induced ubiquitination and degradation of the β 2 AR in
HEK-293 cells. Moreover, siRNA that down-regulates Nedd4 expression inhibited β 2 AR …