Human monocyte chemotactic protein-3 (MCP-3): molecular cloning of the cDNA and comparison with other chemokines
G Opdenakker, G Froyen, P Fiten, P Proost… - Biochemical and …, 1993 - Elsevier
G Opdenakker, G Froyen, P Fiten, P Proost, J Vandamme
Biochemical and biophysical research communications, 1993•ElsevierWhen human MG-63 osteosarcoma cells are triggered with IL-1β, they produce, among
various other cytokines, also monocyte chemotactic proteins (MCPs). Homogeneous MCP-1,
MCP-2 and MCP-3 were found to induce production of gelatinase B and chemotaxis of
monocytes. Based on the almost complete amino acid sequence of natural MCP-3, two sets
of degenerated oligonucleotides were used for the amplification of MCP-3 cDNA. Total RNA
from stimulated MG-63 cells was reverse transcribed and PCRs done on the mRNA: cDNA …
various other cytokines, also monocyte chemotactic proteins (MCPs). Homogeneous MCP-1,
MCP-2 and MCP-3 were found to induce production of gelatinase B and chemotaxis of
monocytes. Based on the almost complete amino acid sequence of natural MCP-3, two sets
of degenerated oligonucleotides were used for the amplification of MCP-3 cDNA. Total RNA
from stimulated MG-63 cells was reverse transcribed and PCRs done on the mRNA: cDNA …
Abstract
When human MG-63 osteosarcoma cells are triggered with IL-1β, they produce, among various other cytokines, also monocyte chemotactic proteins (MCPs). Homogeneous MCP-1, MCP-2 and MCP-3 were found to induce production of gelatinase B and chemotaxis of monocytes. Based on the almost complete amino acid sequence of natural MCP-3, two sets of degenerated oligonucleotides were used for the amplification of MCP-3 cDNA. Total RNA from stimulated MG-63 cells was reverse transcribed and PCRs done on the mRNA:cDNA duplex. Using the PCR-product, several cDNAs were isolated from a cDNA library of IL-1-stimulated MG-63 cells. From the cDNA sequence the complete primary structure of the protein was deduced: MCP-3 shows 71% and 58% amino acid homology with MCP-1 and MCP-2, respectively. Our study establishes MCP-3 as an inflammatory cytokine that regulates macrophage functions. Because MCP-3 is often produced by tumor cell lines and regulates protease secretion by macrophages, its production might also contribute to invasion and metastasis of cancer cells.
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