Existence of only a single functional pool of adenosine triphosphate in human erythrocytes.
E Beutler, E Guinto, W Kuhl… - Proceedings of the …, 1978 - National Acad Sciences
E Beutler, E Guinto, W Kuhl, F Matsumoto
Proceedings of the National Academy of Sciences, 1978•National Acad SciencesThe question of whether separate" membrane" and" soluble" pools of ATP exist in
erythrocytes has been examined. Phosphoglycerate kinase (EC 2.7. 2.3)-derived ("
membrane") ATP was labeled by short-term incubation with inorganic [32P] phosphate.
Pyruvate kinase (EC 2.7. 1.40)-derived (" soluble") ATP is not labeled under these
circumstances. The specific activity of the gamma-phosphate of" soluble" ATP was then
evaluated by the addition of 2-deoxyglucose and measurement of the specific activity of 2 …
erythrocytes has been examined. Phosphoglycerate kinase (EC 2.7. 2.3)-derived ("
membrane") ATP was labeled by short-term incubation with inorganic [32P] phosphate.
Pyruvate kinase (EC 2.7. 1.40)-derived (" soluble") ATP is not labeled under these
circumstances. The specific activity of the gamma-phosphate of" soluble" ATP was then
evaluated by the addition of 2-deoxyglucose and measurement of the specific activity of 2 …
The question of whether separate "membrane" and "soluble" pools of ATP exist in erythrocytes has been examined. Phosphoglycerate kinase (EC 2.7.2.3)-derived ("membrane") ATP was labeled by short-term incubation with inorganic [32P]phosphate. Pyruvate kinase (EC 2.7.1.40)-derived ("soluble")ATP is not labeled under these circumstances. The specific activity of the gamma-phosphate of "soluble" ATP was then evaluated by the addition of 2-deoxyglucose and measurement of the specific activity of 2-deoxyglucose-6-[32P]phosphate formed. This specific activity was essentially the same as the overall specific activity of erythrocyte ATP gama-phosphate, indicating that no functional pools of phosphoglycerate kinase-derived and pyruvate kinase-derived ATP exist in erythrocytes.
National Acad Sciences
