[HTML][HTML] Identification of the components of a glycolytic enzyme metabolon on the human red blood cell membrane
Glycolytic enzymes (GEs) have been shown to exist in multienzyme complexes on the inner
surface of the human erythrocyte membrane. Because no protein other than band 3 has
been found to interact with GEs, and because several GEs do not bind band 3, we decided
to identify the additional membrane proteins that serve as docking sites for GE on the
membrane. For this purpose, a method known as" label transfer" that employs a
photoactivatable trifunctional cross-linking reagent to deliver a biotin from a derivatized GE …
surface of the human erythrocyte membrane. Because no protein other than band 3 has
been found to interact with GEs, and because several GEs do not bind band 3, we decided
to identify the additional membrane proteins that serve as docking sites for GE on the
membrane. For this purpose, a method known as" label transfer" that employs a
photoactivatable trifunctional cross-linking reagent to deliver a biotin from a derivatized GE …