[HTML][HTML] p21-activated kinase 1 phosphorylates and regulates 14-3-3 binding to GEF-H1, a microtubule-localized Rho exchange factor
GEF-H1 is a guanine nucleotide exchange factor for Rho whose activity is regulated through
a cycle of microtubule binding and release. Here we identify a region in the carboxyl
terminus of GEF-H1 that is important for suppression of its guanine nucleotide exchange
activity by microtubules. This portion of the protein includes a coiled-coil motif, a proline-rich
motif that may interact with Src homology 3 domain-containing proteins, and a potential
binding site for 14-3-3 proteins. We identify GEF-H1 as a binding target and substrate for …
a cycle of microtubule binding and release. Here we identify a region in the carboxyl
terminus of GEF-H1 that is important for suppression of its guanine nucleotide exchange
activity by microtubules. This portion of the protein includes a coiled-coil motif, a proline-rich
motif that may interact with Src homology 3 domain-containing proteins, and a potential
binding site for 14-3-3 proteins. We identify GEF-H1 as a binding target and substrate for …
