[HTML][HTML] A genome-wide short hairpin RNA screening of jurkat T-cells for human proteins contributing to productive HIV-1 replication

ML Yeung, L Houzet, VSRK Yedavalli… - Journal of Biological …, 2009 - ASBMB
Short interfering RNAs (siRNAs) have been used to inhibit HIV-1 replication. The durable
inhibition of HIV-1 replication by RNA interference has been impeded, however, by a high
mutation rate when viral sequences are targeted and by cytotoxicity when cellular genes are
knocked down. To identify cellular proteins that contribute to HIV-1 replication that can be
chronically silenced without significant cytotoxicity, we employed a shRNA library that
targets 54,509 human transcripts. We used this library to select a comprehensive population …