Analysis of renal cell carcinoma as a first step for developing mass spectrometry-based diagnostics

K Yoshimura, LC Chen, MK Mandal… - Journal of The …, 2012 - ACS Publications
K Yoshimura, LC Chen, MK Mandal, T Nakazawa, Z Yu, T Uchiyama, H Hori, K Tanabe
Journal of The American Society for Mass Spectrometry, 2012ACS Publications
Immediate diagnosis of human specimen is an essential prerequisites in medical routines.
This study aimed to establish a novel cancer diagnostics system based on probe
electrospray ionization-mass spectrometry (PESI-MS) combined with statistical data
processing. PESI-MS uses a very fine acupuncture needle as a probe for sampling as well
as for ionization. To demonstrate the applicability of PESI-MS for cancer diagnosis, we
analyzed nine cases of clear cell renal cell carcinoma (ccRCC) by PESI-MS and processed …
Immediate diagnosis of human specimen is an essential prerequisites in medical routines. This study aimed to establish a novel cancer diagnostics system based on probe electrospray ionization-mass spectrometry (PESI-MS) combined with statistical data processing. PESI-MS uses a very fine acupuncture needle as a probe for sampling as well as for ionization. To demonstrate the applicability of PESI-MS for cancer diagnosis, we analyzed nine cases of clear cell renal cell carcinoma (ccRCC) by PESI-MS and processed the data by principal components analysis (PCA). Our system successfully delineated the differences in lipid composition between non-cancerous and cancerous regions. In this case, triacylglycerol (TAG) was reproducibly detected in the cancerous tissue of nine different individuals, the result being consistent with well-known profiles of ccRCC. Moreover, this system enabled us to detect the boundaries of cancerous regions based on the expression of TAG. These results strongly suggest that PESI-MS will be applicable to cancer diagnosis, especially when the number of data is augmented.
ACS Publications