[HTML][HTML] Three RNA polymerase II carboxyl-terminal domain kinases display distinct substrate preferences
Y Ramanathan, SM Rajpara, SM Reza, E Lees… - Journal of Biological …, 2001 - ASBMB
CDK7, CDK8, and CDK9 are cyclin-dependent kinases (CDKs) that phosphorylate the C-
terminal domain (CTD) of RNA polymerase II. They have distinct functions in transcription.
Because the three CDKs target only serine 5 in the heptad repeat of model CTD substrates
containing various numbers of repeats, we tested the hypothesis that the kinases differ in
their ability to phosphorylate CTD heptad arrays. Our data show that the kinases display
different preferences for phosphorylating individual heptads in a synthetic CTD substrate …
terminal domain (CTD) of RNA polymerase II. They have distinct functions in transcription.
Because the three CDKs target only serine 5 in the heptad repeat of model CTD substrates
containing various numbers of repeats, we tested the hypothesis that the kinases differ in
their ability to phosphorylate CTD heptad arrays. Our data show that the kinases display
different preferences for phosphorylating individual heptads in a synthetic CTD substrate …