[HTML][HTML] Lysine 63-linked ubiquitination modulates mixed lineage kinase-3 interaction with JIP1 scaffold protein in cytokine-induced pancreatic β cell death
RK Humphrey, SMA Yu, A Bellary, S Gonuguntla… - Journal of Biological …, 2013 - ASBMB
The mixed lineage kinase MLK3 plays a crucial role in compromising mitochondrial integrity
and functions as a proapoptotic competence factor in the early stages of cytokine-induced
pancreatic β cell death. In an effort to identify mechanisms that regulate MLK3 activity in β
cells, we discovered that IL-1β stimulates Lys-63-linked ubiquitination of MLK3 via a
conserved, TRAF6-binding peptapeptide motif in the catalytic domain of the kinase. TRAF6-
mediated ubiquitination was required for dissociation of inactive monomeric MLK3 from the …
and functions as a proapoptotic competence factor in the early stages of cytokine-induced
pancreatic β cell death. In an effort to identify mechanisms that regulate MLK3 activity in β
cells, we discovered that IL-1β stimulates Lys-63-linked ubiquitination of MLK3 via a
conserved, TRAF6-binding peptapeptide motif in the catalytic domain of the kinase. TRAF6-
mediated ubiquitination was required for dissociation of inactive monomeric MLK3 from the …