Distribution of Smad mRNA and proteins in the rat brain
T Nakajima, R Hata, Y Kunieda, T Kondo - Journal of Chemical …, 2018 - Elsevier
Smad proteins are known to transduce the action of TGF-β superfamily proteins including
TGF-βs, activins, and bone morphogenetic proteins (BMPs). In this study, we examined the
expression of Smad1,-2,-3,-4,-5, and-8 mRNA in the rat brain by means of RT-PCR and in
situ hybridization (ISH). In addition, we examined the nuclear accumulation of Smad1,-2,-3,-
5, and-8 proteins after intracerebroventricular injection of TGF-β1, activin A, or BMP6 with
immunohistochemistry to investigate whether TGF-β, activin, and/or BMP activate Smads in …
TGF-βs, activins, and bone morphogenetic proteins (BMPs). In this study, we examined the
expression of Smad1,-2,-3,-4,-5, and-8 mRNA in the rat brain by means of RT-PCR and in
situ hybridization (ISH). In addition, we examined the nuclear accumulation of Smad1,-2,-3,-
5, and-8 proteins after intracerebroventricular injection of TGF-β1, activin A, or BMP6 with
immunohistochemistry to investigate whether TGF-β, activin, and/or BMP activate Smads in …