Acrolein is a highly reactive α, β-unsaturated aldehyde, but the factors that control its
reactions with nucleophilic groups on proteins remain poorly understood. Lipid peroxidation
and threonine oxidation by myeloperoxidase are potential sources of acrolein during
inflammation. Because both pathways are implicated in atherogenesis and high density
lipoprotein (HDL) is anti-atherogenic, we investigated the possibility that acrolein might
target the major protein of HDL, apolipoprotein AI (apoA-I), for modification. Tandem mass …