Phage display and selections on cells
K Even-Desrumeaux, P Chames - Antibody Engineering: Methods and …, 2012 - Springer
K Even-Desrumeaux, P Chames
Antibody Engineering: Methods and Protocols, Second Edition, 2012•SpringerTraditional methods of phage display panning bind purified antigen to plates or other solid
phases to which libraries are then applied. These methods are not directly applicable to
antigens in their native environment on cell surfaces or in settings where the target antigen
is unknown. We describe here a procedure of a panning strategy on cell surface receptors
including a depletion step. We explain every step of the protocol: production of phage
library, depletion and selection, elution, screening by ELISA, and analysis of soluble …
phases to which libraries are then applied. These methods are not directly applicable to
antigens in their native environment on cell surfaces or in settings where the target antigen
is unknown. We describe here a procedure of a panning strategy on cell surface receptors
including a depletion step. We explain every step of the protocol: production of phage
library, depletion and selection, elution, screening by ELISA, and analysis of soluble …
Abstract
Traditional methods of phage display panning bind purified antigen to plates or other solid phases to which libraries are then applied. These methods are not directly applicable to antigens in their native environment on cell surfaces or in settings where the target antigen is unknown. We describe here a procedure of a panning strategy on cell surface receptors including a depletion step. We explain every step of the protocol: production of phage library, depletion and selection, elution, screening by ELISA, and analysis of soluble antibodies by ELISA and flow cytometry. Finally, several possible variants of the protocol are explained in Subheading 4.
Springer
