Isolation and characterization of an apoprotein from the d< 1.006 lipoproteins of human and canine lymph homologous with the rat A-IV apoprotein
KH Weisgraber, TP Bersot, RW Mahley - Biochemical and Biophysical …, 1978 - Elsevier
KH Weisgraber, TP Bersot, RW Mahley
Biochemical and Biophysical Research Communications, 1978•ElsevierA comparative study of the apoproteins of the d< 1.006 lipoproteins from human, canine, and
rat lymph revealed striking similarities including the presence of an apoprotein apparently
homologous to the 46,000 molecular weight A-IV apoprotein of the rat. Homology was based
on similarities in molecular weight and amino acid composition and on immunochemical
cross-reactivity of the human and canine apoproteins. In contrast to the dog and rat, the
human A-IV equivalent was not a major plasma lipoprotein apoprotein. Another 46,000 …
rat lymph revealed striking similarities including the presence of an apoprotein apparently
homologous to the 46,000 molecular weight A-IV apoprotein of the rat. Homology was based
on similarities in molecular weight and amino acid composition and on immunochemical
cross-reactivity of the human and canine apoproteins. In contrast to the dog and rat, the
human A-IV equivalent was not a major plasma lipoprotein apoprotein. Another 46,000 …
Summary
A comparative study of the apoproteins of the d<1.006 lipoproteins from human, canine, and rat lymph revealed striking similarities including the presence of an apoprotein apparently homologous to the 46,000 molecular weight A-IV apoprotein of the rat. Homology was based on similarities in molecular weight and amino acid composition and on immunochemical cross-reactivity of the human and canine apoproteins. In contrast to the dog and rat, the human A-IV equivalent was not a major plasma lipoprotein apoprotein. Another 46,000 molecular weight apoprotein, apo(E—A-II) complex, was present in certain human plasma lipoproteins but was easily distinguished from apo-A-IV by its reducibility to the E and A-II subunits following mercaptoethanol treatment. Apo-A-IV was not affected by this treatment.
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