[HTML][HTML] Reversible major histocompatibility complex I-peptide multimers containing Ni2+-nitrilotriacetic acid peptides and histidine tags improve analysis and sorting …
J Schmidt, P Guillaume, M Irving… - Journal of Biological …, 2011 - ASBMB
MHC-peptide multimers containing biotinylated MHC-peptide complexes bound to
phycoerythrin (PE) streptavidin (SA) are widely used for analyzing and sorting antigen-
specific T cells. Here we describe alternative T cell-staining reagents that are superior to
conventional reagents. They are built on reversible chelate complexes of Ni 2+-
nitrilotriacetic acid (NTA) with oligohistidines. We synthesized biotinylated linear mono-, di-,
and tetra-NTA compounds using conventional solid phase peptide chemistry and studied …
phycoerythrin (PE) streptavidin (SA) are widely used for analyzing and sorting antigen-
specific T cells. Here we describe alternative T cell-staining reagents that are superior to
conventional reagents. They are built on reversible chelate complexes of Ni 2+-
nitrilotriacetic acid (NTA) with oligohistidines. We synthesized biotinylated linear mono-, di-,
and tetra-NTA compounds using conventional solid phase peptide chemistry and studied …