ADAM 12 cleaves extracellular matrix proteins and correlates with cancer status and stage
ADAM 12 is a member of a family of disintegrin-containing metalloproteases that have been
implicated in a variety of diseases including Alzheimer's disease, arthritis, and cancer. We
purified ADAM 12 from the urine of breast cancer patients via Q-Sepharose anion exchange
and gelatin-Sepharose affinity chromatography followed by protein identification by matrix-
assisted laser desorption/ionization-time of flight mass spectrometry. Four peptides were
identified that spanned the amino acid sequence of ADAM 12. Immunoblot analysis using …
implicated in a variety of diseases including Alzheimer's disease, arthritis, and cancer. We
purified ADAM 12 from the urine of breast cancer patients via Q-Sepharose anion exchange
and gelatin-Sepharose affinity chromatography followed by protein identification by matrix-
assisted laser desorption/ionization-time of flight mass spectrometry. Four peptides were
identified that spanned the amino acid sequence of ADAM 12. Immunoblot analysis using …
