Multispectral tracing in densely labeled mouse brain with nTracer
DH Roossien, BV Sadis, Y Yan, JM Webb… - …, 2019 - academic.oup.com
Bioinformatics, 2019•academic.oup.com
This note describes nTracer, an ImageJ plug-in for user-guided, semi-automated tracing of
multispectral fluorescent tissue samples. This approach allows for rapid and accurate
reconstruction of whole cell morphology of large neuronal populations in densely labeled
brains. Availability and implementation nTracer was written as a plug-in for the open source
image processing software ImageJ. The software, instructional documentation, tutorial
videos, sample image and sample tracing results are available at https://www. cai-lab …
multispectral fluorescent tissue samples. This approach allows for rapid and accurate
reconstruction of whole cell morphology of large neuronal populations in densely labeled
brains. Availability and implementation nTracer was written as a plug-in for the open source
image processing software ImageJ. The software, instructional documentation, tutorial
videos, sample image and sample tracing results are available at https://www. cai-lab …
Summary
This note describes nTracer, an ImageJ plug-in for user-guided, semi-automated tracing of multispectral fluorescent tissue samples. This approach allows for rapid and accurate reconstruction of whole cell morphology of large neuronal populations in densely labeled brains.
Availability and implementation
nTracer was written as a plug-in for the open source image processing software ImageJ. The software, instructional documentation, tutorial videos, sample image and sample tracing results are available at https://www.cai-lab.org/ntracer-tutorial.
Supplementary information
Supplementary data are available at Bioinformatics online.
Oxford University Press