Standard sample collections for blood ghrelin measurements

H Hosoda, K Kangawa - Methods in enzymology, 2012 - Elsevier
Background: Octanoyl modification of ghrelin is rapidly hydrolyzed to des-acyl ghrelin in
blood samples. Owing to the increased interest in ghrelin measurement, a standardized
method of sample collection is required. Methods: This chapter investigates the effect of a
variety of anticoagulants and storage conditions on ghrelin stability. Experiment 1 evaluates
the effects of anticoagulants on ghrelin measurements. Experiment 2 evaluates the effect of
plasma pH on ghrelin stability. Experiment 3 evaluates the mechanisms of degradation of …