Histological and electron microscope staining for the identification of elastic fiber networks

EC Davis, L Li - Fibrosis: Methods and Protocols, 2017 - Springer
EC Davis, L Li
Fibrosis: Methods and Protocols, 2017Springer
Elastic fibers are a major component of the extracellular matrix and are present in many
tissues. Routine histology and standard electron microscopy procedures often do not allow
for clear identification of elastic fibers making their organization and ultrastructure difficult to
study. In this paper, we describe staining methods and procedures to enhance the contrast
of elastin at both the light and electron microscope levels.
Abstract
Elastic fibers are a major component of the extracellular matrix and are present in many tissues. Routine histology and standard electron microscopy procedures often do not allow for clear identification of elastic fibers making their organization and ultrastructure difficult to study. In this paper, we describe staining methods and procedures to enhance the contrast of elastin at both the light and electron microscope levels.
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