Characterization of anti-mouse interleukin-6 receptor antibody
M Okazaki, Y Yamada, N Nishimoto, K Yoshizaki… - Immunology letters, 2002 - Elsevier
Hybridoma that produces rat anti-mouse interleukin 6 receptor (IL-6R) antibody, MR16-1,
was established by the fusion of mouse P3U1 myeloma cells and spleen cells from mouse
soluble IL-6R (sIL-6R)-immunized Wistar rat. In the present study, we examined the
characteristics of MR16-1 in vitro and in vivo. MR16-1 bound to mouse sIL-6R dose-
dependently. MR16-1 suppressed IL-6-induced proliferation of 7TD1 cells in a dose-
dependent manner and this inhibitory effect was reversed by the addition of a higher …
was established by the fusion of mouse P3U1 myeloma cells and spleen cells from mouse
soluble IL-6R (sIL-6R)-immunized Wistar rat. In the present study, we examined the
characteristics of MR16-1 in vitro and in vivo. MR16-1 bound to mouse sIL-6R dose-
dependently. MR16-1 suppressed IL-6-induced proliferation of 7TD1 cells in a dose-
dependent manner and this inhibitory effect was reversed by the addition of a higher …