The protein G 0/G 1 switch gene 2 (G0S2) is a small basic protein that functions as an
endogenous inhibitor of adipose triglyceride lipase (ATGL), a key enzyme in intracellular
lipolysis. In this study, we identified a short sequence covering residues Lys-20 to Ala-52 in
G0S2 that is still fully capable of inhibiting mouse and human ATGL. We found that a
synthetic peptide corresponding to this region inhibits ATGL in a noncompetitive manner in
the nanomolar range. This peptide is highly selective for ATGL and does not inhibit other …