[HTML][HTML] CGI-58/ABHD5 is a coenzyme A-dependent lysophosphatidic acid acyltransferase
G Montero-Moran, JM Caviglia, D McMahon… - Journal of lipid …, 2010 - ASBMB
Mutations in human CGI-58/ABHD5 cause Chanarin-Dorfman syndrome (CDS),
characterized by excessive storage of triacylglycerol in tissues. CGI-58 is an α/β-hydrolase
fold enzyme expressed in all vertebrates. The carboxyl terminus includes a highly conserved
consensus sequence (HXXXXD) for acyltransferase activity. Mouse CGI-58 was expressed
in Escherichia coli as a fusion protein with two amino terminal 6-histidine tags. Recombinant
CGI-58 displayed acyl-CoA-dependent acyltransferase activity to lysophosphatidic acid, but …
characterized by excessive storage of triacylglycerol in tissues. CGI-58 is an α/β-hydrolase
fold enzyme expressed in all vertebrates. The carboxyl terminus includes a highly conserved
consensus sequence (HXXXXD) for acyltransferase activity. Mouse CGI-58 was expressed
in Escherichia coli as a fusion protein with two amino terminal 6-histidine tags. Recombinant
CGI-58 displayed acyl-CoA-dependent acyltransferase activity to lysophosphatidic acid, but …