Hypoxia regulates the expression of fatty acid–binding proteins in primary term human trophoblasts

T Biron-Shental, WT Schaiff, CK Ratajczak… - American journal of …, 2007 - Elsevier
OBJECTIVE: Fatty acids (FAs) are essential for fetal development. Cellular FA uptake is
modulated by fatty acid–binding proteins (FABPs). We hypothesized that hypoxia regulates
the expression of FABPs in human trophoblasts. STUDY DESIGN: Primary term human
trophoblasts were cultured for 72 hours in either standard (O2= 20%) or hypoxic (O2< 1%)
conditions. FABP expression was interrogated using polymerase chain reaction and
Western immunoblotting. Trophoblast lipid droplets were examined using dipyrromethene …