Combinatorial alanine-scanning
KL Morrison, GA Weiss - Current opinion in chemical biology, 2001 - Elsevier
KL Morrison, GA Weiss
Current opinion in chemical biology, 2001•ElsevierCombinatorial libraries of alanine-substituted proteins can be used to rapidly identify
residues important for protein function, stability and shape. Each alanine substitution
examines the contribution of an individual amino acid sidechain to the functionality of the
protein. The recently described method of shotgun scanning uses phage-displayed libraries
of alanine-substituted proteins for high-throughput analysis.
residues important for protein function, stability and shape. Each alanine substitution
examines the contribution of an individual amino acid sidechain to the functionality of the
protein. The recently described method of shotgun scanning uses phage-displayed libraries
of alanine-substituted proteins for high-throughput analysis.
Combinatorial libraries of alanine-substituted proteins can be used to rapidly identify residues important for protein function, stability and shape. Each alanine substitution examines the contribution of an individual amino acid sidechain to the functionality of the protein. The recently described method of shotgun scanning uses phage-displayed libraries of alanine-substituted proteins for high-throughput analysis.
Elsevier
