DADA2: High-resolution sample inference from Illumina amplicon data
Nature methods, 2016•nature.com
We present the open-source software package DADA2 for modeling and correcting Illumina-
sequenced amplicon errors (https://github. com/benjjneb/dada2). DADA2 infers sample
sequences exactly and resolves differences of as little as 1 nucleotide. In several mock
communities, DADA2 identified more real variants and output fewer spurious sequences
than other methods. We applied DADA2 to vaginal samples from a cohort of pregnant
women, revealing a diversity of previously undetected Lactobacillus crispatus variants.
sequenced amplicon errors (https://github. com/benjjneb/dada2). DADA2 infers sample
sequences exactly and resolves differences of as little as 1 nucleotide. In several mock
communities, DADA2 identified more real variants and output fewer spurious sequences
than other methods. We applied DADA2 to vaginal samples from a cohort of pregnant
women, revealing a diversity of previously undetected Lactobacillus crispatus variants.
Abstract
We present the open-source software package DADA2 for modeling and correcting Illumina-sequenced amplicon errors (https://github.com/benjjneb/dada2). DADA2 infers sample sequences exactly and resolves differences of as little as 1 nucleotide. In several mock communities, DADA2 identified more real variants and output fewer spurious sequences than other methods. We applied DADA2 to vaginal samples from a cohort of pregnant women, revealing a diversity of previously undetected Lactobacillus crispatus variants.
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