TALENoffer: genome-wide TALEN off-target prediction
Transcription activator-like effector nucleases (TALENs) have become an accepted tool for
targeted mutagenesis, but undesired off-targets remain an important issue. We present
TALENoffer, a novel tool for the genome-wide prediction of TALEN off-targets. We show that
TALENoffer successfully predicts known off-targets of engineered TALENs and yields a
competitive runtime, scanning complete mammalian genomes within a few minutes.
Availability: TALENoffer is available as a command line program from http://www. jstacs …
targeted mutagenesis, but undesired off-targets remain an important issue. We present
TALENoffer, a novel tool for the genome-wide prediction of TALEN off-targets. We show that
TALENoffer successfully predicts known off-targets of engineered TALENs and yields a
competitive runtime, scanning complete mammalian genomes within a few minutes.
Availability: TALENoffer is available as a command line program from http://www. jstacs …
Abstract
Summary: Transcription activator-like effector nucleases (TALENs) have become an accepted tool for targeted mutagenesis, but undesired off-targets remain an important issue. We present TALENoffer, a novel tool for the genome-wide prediction of TALEN off-targets. We show that TALENoffer successfully predicts known off-targets of engineered TALENs and yields a competitive runtime, scanning complete mammalian genomes within a few minutes.
Availability: TALENoffer is available as a command line program from http://www.jstacs.de/index.php/TALENoffer and as a Galaxy server at http://galaxy.informatik.uni-halle.de.
Contact: grau@informatik.uni-halle.de
Supplementary Information: Supplementary data are available at Bioinformatics online.
Oxford University Press