A novel and efficient method for culturing mouse nucleus pulposus cells
J Kushioka, T Kaito, R Chijimatsu, R Okada, H Ishiguro… - The Spine Journal, 2019 - Elsevier
BACKGROUND CONTEXT As degeneration of the nucleus pulposus (NP) is a major cause
of intervertebral disc degeneration, research directed toward nucleus pulposus cells (NPCs)
is drawing increased attention. However, caused by the difficulties associated with their
harvest and culture, there are few reports describing cultivation methods for mouse NP cells
(mNPCs). PURPOSE To establish efficient culture methods for mNPCs. STUDY DESIGN In
vitro animal study. METHODS After primary 3-dimensional (3D) gel culture of mNPCs and …
of intervertebral disc degeneration, research directed toward nucleus pulposus cells (NPCs)
is drawing increased attention. However, caused by the difficulties associated with their
harvest and culture, there are few reports describing cultivation methods for mouse NP cells
(mNPCs). PURPOSE To establish efficient culture methods for mNPCs. STUDY DESIGN In
vitro animal study. METHODS After primary 3-dimensional (3D) gel culture of mNPCs and …
