Increased number of CD25+ FoxP3+ regulatory T cells in oral squamous cell carcinomas detected by chromogenic immunohistochemical double staining
S Schwarz, M Butz, C Morsczeck… - Journal of oral …, 2008 - Wiley Online Library
S Schwarz, M Butz, C Morsczeck, TE Reichert, O Driemel
Journal of oral pathology & medicine, 2008•Wiley Online LibraryBackground: The role of tumor‐infiltrating regulatory T cells (Treg) compromising antitumor
effects of immune cells in oral squamous cell carcinoma (OSCC) is largely unknown.
Purpose: The presence of CD25+ FoxP3+ Treg as well as of CD3+ FoxP3+ and of CD8+
FoxP3+ tumor‐infiltrating lymphocytes (TIL) was verified in OSCC and compared with non‐
cancerous lymphoepithelial tissue. Method: Three double stainings (CD3/FoxP3,
CD8/FoxP3 and CD25/FoxP3) were performed on tissue sections of 15 OSCC and …
effects of immune cells in oral squamous cell carcinoma (OSCC) is largely unknown.
Purpose: The presence of CD25+ FoxP3+ Treg as well as of CD3+ FoxP3+ and of CD8+
FoxP3+ tumor‐infiltrating lymphocytes (TIL) was verified in OSCC and compared with non‐
cancerous lymphoepithelial tissue. Method: Three double stainings (CD3/FoxP3,
CD8/FoxP3 and CD25/FoxP3) were performed on tissue sections of 15 OSCC and …
Background: The role of tumor‐infiltrating regulatory T cells (Treg) compromising antitumor effects of immune cells in oral squamous cell carcinoma (OSCC) is largely unknown.
Purpose: The presence of CD25+ FoxP3+ Treg as well as of CD3+ FoxP3+ and of CD8+ FoxP3+ tumor‐infiltrating lymphocytes (TIL) was verified in OSCC and compared with non‐cancerous lymphoepithelial tissue.
Method: Three double stainings (CD3/FoxP3, CD8/FoxP3 and CD25/FoxP3) were performed on tissue sections of 15 OSCC and compared with 15 human tonsils.
Results: OSCC biopsy samples provide evidence for a strong infiltration of TIL, in particular, naturally occurring CD25+ FoxP3+ Treg. Whereas a comparison of OSCC and control tissue did not show significant changes in the number of CD3+ FoxP3+ TIL and of CD8+ FoxP3+ TIL, a significantly higher frequency of CD25+ FoxP3+ TIL (Treg) could be observed in OSCC (P < 0.001, two‐sided t‐test). Given the small number of specimens, a significant correlation with tumor stage could not be verified.
Conclusion: Chromogenic double staining of CD4/FoxP3 is a promising tool for the detection of Treg in paraffin‐embedded tissue of OSCC.
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