Alterations of cell monolayer integrity and increased vascular permeability are key to many
pathologies, including atherosclerosis, stroke, lung injury, cancer, digestive disorders and
others. Current approaches to probe cell permeability require specific culture conditions and
provide an average estimation of trans-monolayer permeability, while analysis of regional
monolayer permeability in static and mechanically challenged monolayer at a single-cell
scale resolution remains unavailable. We describe a novel method for visualization and …