The recognition of the importance of mRNA turnover in regulating eukaryotic gene
expression has mandated the development of reliable, rigorous, and “user‐friendly”
methods to accurately measure changes in mRNA stability in mammalian cells. Frequently,
mRNA stability is studied indirectly by analyzing the steady‐state level of mRNA in the
cytoplasm; in this case, changes in mRNA abundance are assumed to reflect only mRNA
degradation, an assumption that is not always correct. Although direct measurements of …