Use of a selective inhibitor of liver carnitine palmitoyltransferase I (CPT I) allows quantification of its contribution to total CPT I activity in rat heart. Evidence that the …
BC Weis, AT Cowan, N Brown, DW Foster… - Journal of Biological …, 1994 - ASBMB
It has recently been established that rat heart mitochondria contain two isoforms of carnitine
palmitoyltransferase I (CPT I), the minor 88-kDa variant being identical to liver CPT I (L-CPT
I) and the dominant 82-kDa form resembling the skeletal muscle enzyme (M-CPT I)(Weis,
BC, Esser, V., Foster, DW, and McGarry, JD (1994) J. Biol. Chem. 269, 18712-18715). To
quantify the functional contribution of L-CPT I to overall CPT I activity in heart mitochondria a
selective inhibitor of the former was needed. The dinitrophenol analog of 2 [6-(4 …
palmitoyltransferase I (CPT I), the minor 88-kDa variant being identical to liver CPT I (L-CPT
I) and the dominant 82-kDa form resembling the skeletal muscle enzyme (M-CPT I)(Weis,
BC, Esser, V., Foster, DW, and McGarry, JD (1994) J. Biol. Chem. 269, 18712-18715). To
quantify the functional contribution of L-CPT I to overall CPT I activity in heart mitochondria a
selective inhibitor of the former was needed. The dinitrophenol analog of 2 [6-(4 …